Anatomical Studies of Alfalfa Accessions under Normal and Stress

Author(s)

Rafia Zia , Sidra Ahmad , Humera Razzaq , Rimsha Azam , Izwa shafique ,

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Volume 10 - April 2021 (04)

Abstract

Abiotic stresses have negative impact upon the plant growth and development.  Among abiotic stresses salinity is the most widespread issue. It adversely affects the physiological activities of plants.Salt stress negatively affects alfalfa (Medicago sativa L.) production. Salt stress is one of the main environmental strains that effects the plants development and reduces the yield. Stress also affects the plants at cellular level because it causes toxicity. Pakistan is one of the major salt effected countries in the world. Salinity reduces seed germination rate. To investigate the salt stress tolerance among alfalfa morphological and yield related components an experiment was carried out in 2019-2020. The Research was conducted in complete randomized design by using exotic and indigenous four genotypes (V1, V2, V3 and V4) were  grown under three salt stress treatment control (T0) 5ml (T1) 120ml (T2) 140ml at research area of Department of Plant Breeding and Genetics, University of Agriculture Faisalabad. Supply of different treatments of salt stress accelerated the seedling growth and increase tolerance in alfalfa to stress.The impacts of salt treatments on morphological traits ( Germination percentage (%), Fresh root weight (g), Dry root weight (g), Fresh shoot weight (g), Dry shoot weight (g), Root length (cm), Shoot length (cm), Number of leaves, Fresh seedling weight (g), Dry seedling weight (g)) were analyzed by CRD, ANOVA factorial analysis and correlation-coefficient analysis. The information gained could be used in selection and breeding program aimed to improve alfalfa.

Keywords

Seedling, Nitrogen fixation, Salt stress, Rhizobium symbiosis.  

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