Anatomical Studies of Alfalfa Accessions under Normal and Stress


Rafia Zia , Sidra Ahmad , Humera Razzaq , Rimsha Azam , Izwa shafique ,

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Volume 10 - April 2021 (04)


Abiotic stresses have negative impact upon the plant growth and development.  Among abiotic stresses salinity is the most widespread issue. It adversely affects the physiological activities of plants.Salt stress negatively affects alfalfa (Medicago sativa L.) production. Salt stress is one of the main environmental strains that effects the plants development and reduces the yield. Stress also affects the plants at cellular level because it causes toxicity. Pakistan is one of the major salt effected countries in the world. Salinity reduces seed germination rate. To investigate the salt stress tolerance among alfalfa morphological and yield related components an experiment was carried out in 2019-2020. The Research was conducted in complete randomized design by using exotic and indigenous four genotypes (V1, V2, V3 and V4) were  grown under three salt stress treatment control (T0) 5ml (T1) 120ml (T2) 140ml at research area of Department of Plant Breeding and Genetics, University of Agriculture Faisalabad. Supply of different treatments of salt stress accelerated the seedling growth and increase tolerance in alfalfa to stress.The impacts of salt treatments on morphological traits ( Germination percentage (%), Fresh root weight (g), Dry root weight (g), Fresh shoot weight (g), Dry shoot weight (g), Root length (cm), Shoot length (cm), Number of leaves, Fresh seedling weight (g), Dry seedling weight (g)) were analyzed by CRD, ANOVA factorial analysis and correlation-coefficient analysis. The information gained could be used in selection and breeding program aimed to improve alfalfa.


Seedling, Nitrogen fixation, Salt stress, Rhizobium symbiosis.  


                       i            Becana M, Dalton DA, Moran JF, Iturbe‐Ormaetxe I, Matamoros MA, Rubio CM . 2000.Reactive oxygen species and antioxidants in legume nodules. Physiol Plant 109:372–381.

        ii            Bolanos-Aguilar ED, Huyghe C, Ecalle J, Hacquet C, Julier B 2002. Effect of cultivar and environment on seed yield in alfalfa. Crop Science 42:45-50.

      iii            Ertani A, Schiavon M, Muscolo A, Nardi S. 2013. Alfalfa plant derived biostimulant stimulate short-term growth of salt stressed Zea mays L. plants. Plant Soil 364:145–158.

       iv            Evelin H, Kapoor R, Giri B. 2009.Arbuscular mycorrhizal fungi in alleviation of salt stress: a review. Ann Bot 104:1263–1280

         v            Grover M, Ali SZ, Sandhya V, Rasul A, Venkateswarlu B. 2011. Role of microorganisms in adaptation of agriculture crops to abiotic stresses. World J Microbiol Biotechnol 27:1231–1240.

       vi            Kiers ET, Rousseau RA, West SA, Denison RF. 2003. Host sanctions and the legume-rhizobium mutualism. Nature 425:78–81

     vii            Porcel R, Aroca R, Ruiz Lozano JM. 2012. Salinity stress alleviation using arbuscular mycorrhizal fungi. A review. Agron Sustainable Dev 32:181–200

   viii            Postnikova, O. A., J. Shao and L. G. Nemchinov. 2013. Analysis of the alfalfa root transcriptome in response to salinity stress. Plant Cell Physiol., 54: 1041-1055.

       ix            Shrivastava P, Kumar R. 2015. Soil salinity: a serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi J Biol Sci 22:123–131.

         x            Yacoubi, R., C. Job, M. Belghazi, W. Chaibi and D. Job. 2013. Proteomic analysis of the enhancement of seed vigour in osmoprimed alfalfa seeds germinated under salinity stress. Seed Sci. Res.,23: 99-110.

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