Method for the Determination of Captopril in Bulk, Pharmaceutical Formulations and Serum by HPLC using two different System.

Author(s)

Safila Naveed , Najma Sultana , M.Saeed Arayne ,

Download Full PDF Pages: 08-14 | Views: 949 | Downloads: 218 | DOI: 10.5281/zenodo.3407999

Volume 2 - March 2013 (03)

Abstract

A simple reversed phase HPLC method have been successfully developed and validated for the quantitative determination of captopril (CAP) in bulk material, pharmaceutical formulation and serum. Purospher Start C18 (250cm x 4.6mm, 5μm) and Hypersil ODS C18 (150×4.6mm, 5micron) columns were used. The mobile phase, (methanol-water 50:50(v/v) pH 3.0 adjusted by phosphoric acid), was delivered at a flow rate of 1mLmin-1, eluent was monitored using UV detector at 215, 220, 225 nm. The proposed method is specific, accurate (99-102%), precise (intra-day variation 0.04-0.98 % and inter-day variation 0.06-1.5%) and linearity (R2>0.999) within the desired range 1.25-50 μgmL-1 concentration. The detection limit and quantification limit was 2.0 ngmL-1 and 8.0 ngmL-1 respectively. The anticipated method is applicable to routine analysis of CAP in pharmaceutical formulations as well as in human serum samples. 

Keywords

Captopril, HPLC, Serum

References

  1. N. D. Jaime, and A.R William. Wilson and Gisvold’s Text Book of Organic Medicinal and Pharmaceutical Chemistry, Lippincott, J.B. Company, 1991,9th ed., 564.
  2. G.K, Bertam. Basis and Clinical Pharmacology. Appleton and Lange,1998,Revised ed, 197-213
  3. T. Huang,Z .He, B. Yang, L .Shao,J. Pharm. Biomed. Anal, 41, (2006) 644.
  4. T .Mirza, Tan, H. S. I.; J. Pharm. Biomed. 25, (2001), 39.
  5. Walily,E.; Razak, O. A.; Belal, S. F.; Bakry ,R. S.; J. Pharm. Biomed. Anal, 21 (1999), 439.
  6. C. Arroyoa, C.L. Calulla, L.G .Capdevilaa, I. Gichb, M. Barbanojb,Bonala. J. Chromatogr. B, 688 (1997),339.
  7. N. Sultana, M. S. Arayne, and S. Naveed; Simultaneous Quantitation of Captopril and NSAID’s in API, DosageFormulations and Human Serum by RP-HPLC Journal of the Chinese Chemical Society, 57(2010) , 62-67
  8. N. Sultana, M. S. Arayne, and S. Naveed; Simultaneous Determination of Captopril and Statins in API,Pharmaceutical Formulations and in Human Serum by RP-HPLC Journal of the Chinese Chemical Society, 57, (2010) 378-383.
  9. P.D. Tzanavaras, D.G, Themelis, A. Economou, G. Theodoridis.Talanta, 57(2002),575.

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